BACTERIAENTERIC PATHOGENS AND ANTIMICROBIAL SUSCEPTIBILITY PROFILE AMONG PEDIATRIC PATIENTS WITH DIARRHEA: A CROSS SECTIONAL STUDY IN SELECTED HEALTH FACILITIES, ADDIS ABABA, ETHIOPIA

Authors

  • Rawleigh Howe AHRI
  • Zeleke Ayenew
  • Habtamu Biazin
  • Solomon Gebre-Selassie
  • Biruk Yeshitila
  • Abrham Assefa
  • Tamrat Abebe

Abstract

Background:  Diarrheal disease remains a major public health problem in developing countries including Ethiopia. The current study was designed to isolate medically important bacterial enteric pathogens and assess the antimicrobial susceptibility testing of bacteria causing diarrhea in pediatrics for those antibiotics were prescribing in Integrated Management of Childhood illness (IMCI).

Methods: Across-sectional study to determine bacterial enteric pathogenic microorganisms that cause diarrhea and antimicrobial susceptibility profile was carried out in selected health facilities of Addis Ababa, Ethiopia from November 2016 to May 2017. Stool specimens from pediatric patients aged 0-14 years were collected randomly from two health centers and one specialized hospital to identify bacterial enteric pathogens. Antimicrobial susceptibility tests were performed on all bacterial isolates using the Kirby-Bauer disc diffusion method.

Results: Out of 290 study patients with diarrhea examined, the majority of bacterial enteropahogens isolated in the study were Shigella spp 22(7.6%) followed by enterohemorrgic E.coli O157:H7 13(4.5%), Salmonella spp 7(2.4%).  Bacterial isolates from diarrheal patients showed antimicrobial resistance to ampicillin, Augmentin, trimethoprim-sulphamethoxazole and ciprfloxacillin. Salmonella spp showed resistance to trimethoprim-sulphamethoxazole and chloramphenicol, 42.9% and 14.3% respectively. Another enteric bacteria Shigella spp were resistant to 77.3%ampicillin, 68.2% trimethoprim-sulphamethoxazole and36.4%Augmentin whereas E.coli O157:H7 resistance anti-biogram showed 69.2%ampicillin,46.1% trimethoprim-sulphamethoxazole,38.5%Augmentin, 23.1%ciprfloxacillin and Amikacin, ceftriaxone and gentamycin were resistant with the same rate  of 15.4% .

 

Key words: Enteric pathogens, diarrhea, antimicrobial susceptibility, pediatrics, Addis Ababa, Ethiopia


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2019-04-12

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