• Daniel Demissie
  • Berhanu Seyoum
  • Melake Demena
  • Biruk Yeshitila


Background: Puerperal sepsis is a major cause of maternal morbidity and mortality and is usually within the first 42 days after child birth and it’s the second cause of maternal morbidity and mortality in the resource poor countries.

Objective:The aim of the study was to assess the prevalence of septicemia, its Bacterial isolates drug susceptibility patterns and associated factors among suspected women attending delivery at Dil-chora referral hospital, Dire Dawa, Eastern Ethiopia from May 1 to July 30 /2016.

Method: Cross sectional study was conducted with a sample size of 441 women of the age group 15-49 years at Dil-chora hospital, Dire Dawa, Eastern Ethiopia from May 1 to July 30 /2016. Socio-demographic and clinical data variables were collected using structured interview questionnaire. Blood was collected aseptically and inoculated aerobically for 48 hours. Antimicrobial susceptibility patternof isolated bacteria was determined by Kirby Bauer disc diffusion method. Data analyzed using SPSS version 16; Binary logistic regression was used to measure the association. Significant variables were further adjusted using multivariate analysis.


Conclusion: The prevalence of septicemia was 12.9%. Coagulase negative staphylococci and E. coli were the predominant isolated bacteria. Most of bacterial isolates were resistant against commonly used antibiotics such as ampicillin, amoxicillin and tetracycline.

Key word: Septicemia, Puerperal sepsis, maternal septicemia, Antimicrobial resistance.




WHO. (2015).managing puerperal sepsis Education material for teachers of midwifery: midwifery education modules. Geneva: World Health Organization.

AbouZahr C, 2013.Global burden of maternal death and disability. British Medical Bulletin, 67:1-11.

Lawn, JE. Cousens S, .Zupan J, 2014.Moternal Survival Steering Team: When? Where? Why? Lancet, 365(9462):891-900.International Statistical Classification of Diseases and Related Health Problems 10th Revision (ICD-10).

WHO. (2004).Essential Medicines Library (EMLib), 2004.Diseases, clinical indications and Symptoms Details Puerperal sepsis WMF edition.

Phillip,R, Mitchell M., Andrew R, 2013. Surviving Sepsis Campaign: International Guidelines for Management of Severe Sepsis and Septic Shock:

WHO. (2010).Estimates of Maternal Mortality: A New Approach by World Health Organization (WHO) and United Nations Children’s Fund (UNICEF)".

Khan KS, Wojdyla D, Say L, Gulmezoglu AM, Van Look PF, 2006.Analysis of causes of maternal death: a systematic review. Lancet, 367(9516):1066-1074.

Ethiopia MDGs report 2012 .Assessing progress towards the millennium development goals Ethiopia MDGs report 2012 December 2012 - Addis Ababa, Ethiopia.

Mulat D, Gizachew Y, Mucheye G ,Alemayehu G, Tigist A and Tinebeb T et al,.2013. Bacterial profile and antimicrobial susceptibility pattern in septicemia suspected patients attending Gondar University Hospital, Northwest Ethiopia .journal of biomedcenteral Research, 6:283

Kramer, H.M.C., Schutte, J.M., Zwart, J.J,2013. Maternal mortality and severe morbidity from sepsis in the Netherlands.Acta. Obstet. Gynecol., 88: 647 53.

Maharaj, D.2007. Puerperal pyrexia: a review, Part I. Obstet. Gynecol. Surv.;62: 393

Mackie and McCartney, 1996. Practical Medical Microbiology.14th edition .Churchill Livingstone, New York.1996; p - 507.

Cheesebrough.M, 2006. District laboratory Practice in tropical countries. MicrobiologyPart II, 2nd ed Cambridge University press, London UK: 105-114

Clinical Laboratory Standard Institute (CLSI), 2014. Performance Standards for Antimicrobial Susceptibility Testing, Twentieth informational supplements, CLSI document M100-S20.Wayne, PA: clinical and laboratory standard Institute.

WHO.(2003). Basic Laboratory Procedures in clinical Bacteriology 2nd Edition.Geneva; World health organization.

WHO. 1994. Mother–baby package: implementing safe motherhood in countries. World HealthOrganization;Geneva:.

Majda Q and Farooq A, 2011. Prevalence of microbial isolates in blood cultures and their antimicrobial susceptibility profiles department of pathology, shalamar medical and dental college, Lahore. Biomedica Vol.27, Jul. – Dec. 2011.Bio-6.

Mohamed I, Mohamed A, Rabie A, 2013. Puerperal Sepsis in Rural Hospital Sudan.Mat Soc Med.; 25(1): 19-22 DOI: 10.5455/msm.25.19-22.

Sabiha S. Tamboli, Saleem B. Tamboli, SunandaShrikhande, 2016 .Puerperal sepsis: predominant organisms and their antibiotic sensitivity pattern, International Journal of Reproduction, Contraception, Obstetrics and Gynecology

Shagufta Q, Kashmi S, Bushra S, Aaliya E, Rana K. Sherwani, Asfia S and Fatima K, 2015. Microbial Profile in Females with Puerperal Sepsis: A Major Threat to Women s Health: Study at a Tertiary Health Care Centre, department of Pathology, Jawaharlal Nehru medical College (JNMC), Aligarh MuslimUniversity (AMU),U.P,India.Int.J.Curr.Microbiol.App.SciSpecialIssue-1:248-255.

Prakash KP, VinodArora, Geethanjali,(2011) Bloodstream Bacterial Pathogens and their Antibiotic Resistance Pattern in Dhahira Region, Oman. Oman Medical Journal Vol. 26, No. 4: 240-247DOI 10. 5001/omj.2011.59

Beagie R, Priscilla L, Elon T, John D, 1975. Perinatal infection and vaginal flora.Am J ObstetGynecol 122: 31-33.

Dillen, J.V., Zwart, J., Schutte, J., Roosmalen, J.V. 2010. Maternal sepsis: epidemiology, etiology and outcome. Curr.Opin. Infect. Dis., 23: 249 54.

Komolafe, A. O. and Adegoke, A.A, 2008. Incidence of bacterial Septicemia in Ile-Ife Metropolis, Nigeria Department of Medical Microbiology and Parasitology, ObafemiAwolowo University Hospital complex, Ile-Ife, Nigeria Malaysian Journal of Microbiology, 4(2)

Volk A, Gebhraddt M, Hammarskjold M and Kadner RJ. 1996. Essentials of medical Microbiology, 5th ed. Lippincott-Raven, Philadelphia: 345-348.

Meenakshi K, Maria S and M.Prasad N, 2014.Bacterial profile of blood stream infections and antibiotic susceptibility pattern of isolates, Int.J.Curr.Microbiol.App.Sci

Melissa E. Bauer, Brian T. Bateman, Samuel T. Bauer, Amy M. Shank, and Jill M. Mhyre, 2013.Maternal sepsis mortality and morbidity during hospitalization for Delivery,Temporal Trends and Independent Associations for Severe Sepsis.; Volume 117, Number 4

Khaskheli MN, Baloch S, Sheeba A.2013. Risk factors and complications of puerperal sepsis at a tertiary healthcare centre. Pak J Med Sci 29(4):972-976.doi:

El-Mahally A, A, Aneer NH. 2014. Risk factors o






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