A Immunohistochemical expression of Bcl-2 and EGFR biomarkers in Urothelial carcinoma of the bladder lesions and its relation to grade
Immunohistochemical expression of Bcl-2 and EGFR biomarkers in Urothelial carcinoma
Keywords:Bcl-2, biomarkers, epidermal growth factor receptor, urothelial carcinoma
Over-expression of Bcl-2 and EGFR is seen in many malignancies. Since few studies have studied these biomarkers in bladder tumors, the aim of this study is to evaluate expression of Bcl-2 and EGFR biomarkers in benign and malignant tumors of urothelial carcinoma of bladder.
This case control study was conducted on 57 bladder tumors (40 malignant tumors and 17 benign tumors) in Shahid Rahnemoun hospital, Yazd, Iran during 2016-2018. Immunohistochemistry method was used for assessment of EGFR and bcl-2 expression in tissue tumors.
Frequency distribution of patients in terms of EGFR showed that among 57 tumors, 10 tumors (17.4%) were EGFR positive. Moreover, among them, 25 tumors were bcl-2 positive (44%). There was no significant difference between benign and malignant tumors in terms of EGFR (p=0.830) and bcl-2 expression (p=0.094). The mean EGFR in low and high grade was 4.13Â± 13.22 and 7.69Â± 26.26, respectively (p= 0.91).The mean bcl-2 in low and high grade was 3.72Â± 12.81 and 13.31Â±26.14, respectively (p=0.51). Moreover, significant difference was seen between two groups (benign and malignant group), regarding age and tumor size (p>0.05).
According to results of current study, no significant difference was seen between benign and malignant tumors in terms of EGFR and bcl-2 expression. Moreover, no relation was seen between EGFR and Bcl2 expression and grade. Therefore, expression of these biomarkers didn't affect on progression of the tumor and clinical outcomes. In addition, malignant or benign tumor was affected by factors including age and tumor size.
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