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BACTERIOLOGY AND RISK FACTORS OF BACTERIAL KERATITIS IN JIMMA, SOUTHWEST ETHIOPIA

Tewelde Tesfaye Gebremariam
Department of Medical Microbiology, Mekelle Universty
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  • Articles
  • Submited: June 1, 2015
  • Published: December 21, 2015

Abstract

Background: In East Africa, particularly in Ethiopia, bacterial keratitis is a major cause of blindness.  

Objective: The aims of this study were to identify risk factors of bacterial keratitis and the spectrum of bacterial etiologies, and to assess the in-vitro antimicrobial susceptibility of these bacterial isolates at Jimma University Specialized Hospital, Southwest Ethiopia.

Methods: A prospective study was employed from January 2012 to June 2012 from which a total of 24 patients with bacterial keratitis were included in the study. Corneal scrapings were collected, transported and microbiologically processed using standard operating procedures.

Results: Four different predisposing factors for bacterial keratitis were identified: corneal trauma 9 (37.5%), blepharitis 7 (29.2%), herpetic keratitis, and use of contaminated medications 4.. Bilateral corneal infection was found in 5 (20.8%) of the cases. A total of 24 corneal scrapings were collected for microbiological evaluation, of which 20 (83%) had bacterial growth. The isolated bacterial pathogens were Pseudomonas aeruginosa 10 (41.7%), Staphyloccus aureus 5 (20.8%), Serratia marcescens 3 (12.5%), followed by Streptococcus pneumoniae 2 (8.3%). Antimicrobial susceptibility testing revealed that 85% of Gram-negative bacilli were susceptible to gentamicin and ciprofloxacin, while 86% of Gram-positive cocci were susceptible to vancomycin and Ciprofloxacin.

Conclusions: Corneal trauma was the most common risk factor for bacterial keratitis followed by blepharitis. Bacteriological analysis of corneal scrapings also revealed that P. aeruginosa was the most common isolate followed by S. aureus; the antibiotic with the highest susceptibility was ciprofloxacin. As drug resistance among bacterial pathogens is an evolving process, routine surveillance and monitoring studies should be conducted to provide an update and most effective empirical treatment for bacterial keratitis.

Keywords: Antibacterial susceptibility, Bacterial pathogens, corneal trauma, Bacterial Keratitis, Jimma, Ethiopia 

 

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References

  1. Khosravi AD, Mehdinejad M, Heidari M: Bacteriologic findings in patients with ocular infection and antibiotic susceptibility patterns of isolated pathogens. Singapore Med J 2007, 48:741-3.
  2. Schaefer F, Bruttin O, Zografos L, Guex-Crosier Y: Bacterial keratitis: a prospective clinical and microbiological study. Br J Ophthalmol 2001, 85:2-847.
  3. Geethakumari PV, Remya R, Girijadevi MS, Reena A: Bacterial keratitis and fungal keratitis in South Kerala: a comparative study. Kerla J Ophthalmol 2011, XXIII:43-46.
  4. Bataineh H, Hammory Q, Khatatba A: Bacterial keratitis: risk factors and causative agents. Sudan JMS 2008, 3:6-10.
  5. Brown L: Resistance to ocular antibiotics: an overview. Clin Exp Optom 2007, 90:258-62.
  6. Burton MJ, Pithuwa J, Okello E, Afwamba I, Onyango JJ, Oates F, Chevallier C, Hall AB: Microbial keratitis in East Africa: why are the outcomes so poor? Ophthalmic Epidemiol 2011, 18:158-63.
  7. Ubani UA: Bacteriology of external ocular infections in Aba, South Eastern Nigeria. Clin Exp Optom 2009, 92:482-9.
  8. Bharathi MJ, Ramakrishnan R, Shivakumar C, Meenakshi R, Lionalraj D: Etiology and antibacterial susceptibility pattern of community-acquired bacterial ocular infections in a tertiary eye care hospital in south India. Indian J Ophthalmol 2010, 58:497-507.
  9. Sharma S: Antibiotic resistance in ocular bacterial pathogens. Indian J Med Microbiol 2011, 29:218-222.
  10. Tananuvat N, Sienglew S, Ausayakhun S: Microbial keratitis leading to admission at Maharaj Nakorn Chiang Mai Hospital. Chiang Mai Med Bull 2004, 43:93-103.
  11. Bourcier T, Thomas F, Borderie V, Chaumeil C, Laroche L: Bacterial keratitis: predisposing factors, clinical and microbiological review of 300 cases. Br J Ophthalmol 2003, 87:834-8.
  12. Bharathi MJ, Ramakrishnan R, Meenakshi R, Shivakumar C, Raj DL: Analysis of the risk factors predisposing to fungal, bacterial & acanthamoeba keratitis in South India. Indian J Med Res 2009, 749-757.
  13. Al-Mujaini A, Al-Kharusi N, Thakral A, Wali UK: Bacterial keratitis: perspective on epidemiology, clinico-pathogenesis, diagnosis and treatment. SQU Med J 2009, 9:184-195.
  14. Wong RLM, Gangwani RA, Yu LWH, Lai JSM: New treatments for bacterial keratitis. J Ophthalmol 2012, 1-7.
  15. Shoja MR, Manaviat M: Epidemiology and outcome of corneal ulcer in Yazd Shahid Sadoughi Hospital. Acta Medica Iranica 2004, 42:136-8.
  16. Tesfaye T, Beyene G, Gelaw Y, Bekele S, Saravanan M: Bacterial profile and antimicrobial susceptibility pattern of external ocular infections in Jimma University Specialized Hospital, Southwest Ethiopia. AJIDM 2013, 1:13-20.
  17. Tobbara KF, Hyndiuk RA: Infections of the eye. Little, Brown and Company: New York. 1995; 55-7.
  18. Cheesbrough M: District laboratory practice in tropical countries. London, Cambridge. 2nd edition 2006. pp.132-5.
  19. Joseph S, Bertino JR: Impact of antibiotic resistance in the management of ocular infections: the role of current and future antibiotics. Clin Ophthalmol 2009, 3:507-21.
  20. Tabbara K, El-Sheikh H, Aabed B: Extended wear contact lens related bacterial keratitis. Br J Ophthalmol 2000, 84:327-8.
  21. Kunimoto DY, Sharma S, Garg P, Gopinathan U, Miller D, Rao GN: Corneal ulceration in the elderly in Hyderabad, south India. Br J ophthalmol 2000, 84:54-59.
  22. Green M, Apel A, Stapleton F: Risk factors and causative organisms in microbial keratitis. Cornea 2008, 27:22-7.
  23. Kunimoto DY, Sharma S, Garg P, Gopinathan U, Miller D, Rao GN: Corneal ulceration in the elderly in Hyderabad, south India. Br J ophthalmol 2000, 84:54-59.
  24. Norina TJ, Raihan S, Bakiah S, Ezanee M, Liza-Sharmini A T, Hazzabah W H: Microbial keratitis: aetiological diagnosis and clinical features in patients admitted to Hospital Universiti Sains Malaysia. Singapore Med J 2008, 49:67-71.
  25. Bharathi MJ, Ramakrishnan R, Vasu S, Meenakshi R, Shivkumar C, Palaniappan R: Epidemiology of bacterial keratitis in a referral centre in South India. Indian J Med Microbiol 2003, 21:239-245.
  26. Bharathi MJ, Ramakrishnan R, Meenakshi R, Padmavathy S, Shivakumar C, Srinivasan M: Microbial keratitis in South India: influence of risk factors, climate, and geographical variation. Ophthalmic Epidemiol 2007, 14:61-69.
  27. Kumar A, Pandya S, Kavathia G, Antala S, Madan M, Javdekar T: Microbial keratitis in Gujarat, Western India: findings from 200 cases. Pan Afr Med J 2011, 10:48.
  28. Raju KV: Bacterial Keratitis. Korean J Ophtalmol 2008, XX.78-83.
  29. Joseph S, Bertino JR: Impact of antibiotic resistance in the management of ocular infections: the role of current and future antibiotics. Clin Ophthalmol 2009, 3:507-21.
  30. Ramesh S, Ramakrishnan R, Bharathi MJ, Amuthan M, Viswanathan S: Prevalence of bacterial pathogens causing ocular infections in South India. Indian J Pathol Microbiol 2010, 53:281-6.
  31. Biradar S, Chandrashekhar DK, Gangane R, Chandrakanth C, Biradar KG, VinodKumar CS: Spectrum of microbial keratitis and antimicrobial susceptibility at tertiary care teaching hospital in north Karnataka. Int J Pharm Biomed Res 2012, 3:117-20.
  32. Al-Zahrani SHM: Bacteria isolated from contact and non contact lens and antibiotic susceptibility patterns of isolated Pseudomonas aeruginosa. Afr. J. Microbiol. Res 2012, 6:7350-6.
  33. Ogbolu DO, Alli OT, Ephraim IE, Olabiyi FA, Daini OA: In-Vitro efficacy of antimicrobial agents used in the treatment of bacterial eye infections in Ibadan, Nigeria. Afr. J. Cln. Exper. Microbiol 2011, 12:124-7.
How to Cite
Gebremariam, T. T. (2015). BACTERIOLOGY AND RISK FACTORS OF BACTERIAL KERATITIS IN JIMMA, SOUTHWEST ETHIOPIA. Ethiopian Medical Journal, 53(4). Retrieved from https://emjema.org/index.php/EMJ/article/view/126

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